Saturday, August 6, 2011
Juno leaves for Jupiter
Monday, June 6, 2011
E. coli Outbreak in the Europe
Europe is currently besieged by a serious outbreak of a rare strain of E. coli foodborne bacteria. One of the largest outbreaks of E. coli ever recorded strikes Germany and several other European countries. An ongoing Escherichia coli O104:H4 bacterial outbreak began in Germany in May 2011. Certain strains of E. coli are a major cause of foodborne illness. The outbreak started after several people were infected with bacteria leading to hemolytic-uremic syndrome in Germany. Hemolytic-uremic syndrome (HUS) is a medical emergency and requires urgent treatment. 19 people have died by 3 June and around 500 had been hospitalised with HUS due to the intensifying outbreak.
Scientists probing the deadly E. coli strain in Europe are finding the bacteria combines a highly poisonous, but common, toxin with a rarely seen "glue" that binds it to a patient's intestines.
It may take months for the global team of researchers to fully understand the characteristics of the bacteria that has killed at least 17 people in Europe and sickened 1,500. But they fear this E. coli strain is the most toxic yet to hit a human population.
Most Escherichia coli or E. coli bacteria are harmless. The strain that is sickening people in Germany and other parts of Europe, known as 0104:H4, is part of a class of bacteria known as Shiga toxin-producing Escherichia coli, or STEC.
This class has the ability to stick to intestinal walls where it pumps out toxins, causing diarrhea and vomiting. In severe cases, it causes hemolytic uremic syndrome or HUS, attacking the kidneys and causing coma, seizure and stroke.
Germany is now reporting 470 cases of HUS. That is absolutely extraordinary, Dr. Robert Tauxe, a foodborne diseases expert at the U.S. Centers for Disease Control and Prevention, told Reuters. The CDC has been working with German health authorities on the case since late last week.
Affected countries
- Germany
- Spain
- Sweden
- Czech Republic
- Denmark
- The Netherlands
- United Kingdom
- Switzerland
- Poland
- United States
- Austria
- France
E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.
In April 2009, a Michigan State University researcher announced he had developed a working vaccine for a strain of E. coli. Mahdi Saeed, professor of epidemiology and infectious disease in MSU's colleges of Veterinary Medicine and Human Medicine, has applied for a patent for his discovery and has made contact with pharmaceutical companies for commercial production.
General Statistics | ||
1 | Cell length | 2 um or 2x10-6 m |
2 | Cell diameter | 0.8 um or 0.8x10-6 m |
3 | Cell total volume | 1x10-15 L or 1x10-18 m3 (other est. at 0.88x10-15 L) |
4 | Cell aqueous volume | 7 x 10-16 L |
5 | Cell surface area | 6x10-12 m2 |
6 | Cell wet weight | 1x10-15 kg or 1x10-12 g |
7 | Cell dry weight | 3.0x10-16 kg or 3.0x10-13 g |
8 | Periplasm volume | 6.5x10-17 L |
9 | Cytoplasm volume | 6.7x10-16 L |
10 | Envelope volume | 1.6x10-16 L |
11 | Nuclear (DNA+protein) volume | 1.6x10-16 L |
12 | Inner Membrane thickness | 8x10-9 m |
13 | Outer Membrane thickness | 8x10-9 - 15x10-9 m |
14 | Periplasm thickness | 1x10-8 m |
15 | Average size of protein | 360 residues |
16 | Average diameter of ave. protein | 5 nm |
17 | Average MW of protein | 40 kD |
18 | Average prot. oligomerization state | 4 proteins/complex |
19 | Average MW of protein entity | 160 kD |
20 | Average size of mRNA | 1100 bases |
21 | Average length of mRNA | 370 nm |
22 | Average MW of all RNAs | 400 kD |
23 | Average MW of single DNA | 3.0x109 D or 3.0x106 kD |
24 | Average MW of all DNA | 7 x 106 kD |
25 | Average length of DNA (chrom.) | 1.55 mm |
26 | Diameter of chromosome | 490 um |
27 | Diameter of condensed chromosome | 17 um |
28 | Spacing between small organics | 3.6 nm/molecule |
29 | Spacing between ions | 2.1 nm/molecule |
30 | Ave. spacing between proteins | 7 nm/molecule |
31 | Spacing between protein entities | 9 nm/molecule |
32 | Mean Velocity of 70 kD protein (cytoplasm) | 3 nm/ms = 3x10-6 m/s |
33 | Mean Velocity of 40 kD protein (cytoplasm) | 5 nm/ms = 5x10-6 m/s |
34 | Mean Velocity of 30 kD protein (cytoplasm) | 7 nm/ms = 7x10-6 m/s |
35 | Mean Velocity of 14 kD protein (cytoplasm) | 10 nm/ms = 10x10-6 m/s |
36 | Mean Velocity of small molecules (cytoplasm) | 50 nm/ms = 5x10-5 m/s |
37 | Mean Velocity of protein in H2O | 27 nm/ms = 2.7x10-5 m/s |
38 | Mean Velocity of small molecules in H2O | 87 nm/ms = 8.7x10-5 m/s |
39 | Concentration of protein in cell | 200-320 mg/mL (5-8 mM) |
40 | Concentration of RNA in cell | 75-120 mg/mL (0.5-0.8 mM) |
41 | Concentration of DNA in cell | 11-18 mg/mL (5 nM) |
42 | Volume occupied by water | 70% |
43 | Volume occupied by protein | 17% |
44 | Volume occupied by all RNA | 6% |
45 | Volume occupied by rRNA | 5% |
46 | Volume occupied by tRNA | 0.8% |
47 | Volume occupied by mRNA | 0.2% |
48 | Volume occupied by DNA | 1% |
49 | Volume occupied by ribosomes | 8% |
50 | Volume occupied by lipid | 3% |
51 | Volume occupied by LPS | 1% |
52 | Volume occupied by murein | 1% |
53 | Volume occupied by glycogen | 1% |
54 | Volume occupied by ions | 0.3% |
55 | Volume occupied by small organics | 1% |
56 | Translation rate | 40 aa/sec |
57 | RNA polymerase transcription rate | 70 nt/sec |
Large Molecule Copy Numbers | ||
1 | Number of cell walls/cell | 1 |
2 | Number of membranes/cell | 2 |
3 | Number of chromosomes/cell | 2.3 (at mid log phase) |
4 | Number of mRNA/cell | 4000 |
5 | Number of rRNA/cell | 18,000 |
6 | Number of tRNA/cell | 200,000 |
7 | Number of all RNA/cell | 222,000 |
8 | Number of polysaccharides/cell | 39,000 |
9 | Number of murein molecules/cell | 240,000-700,000 |
10 | Number of lipopolysaccharide/cell | 600,000 |
11 | Number of lipids/cell | 25,000,000 |
12 | Number of all lipids/cell | 25,000,000 |
13 | Number of phosphatidylethanolamine | 18,500,000 |
14 | Number of phosphatidylglycerol | 5,000,000 |
15 | Number of cardiolipin | 1,200,000 |
16 | Number of phosphatidylserine | 500,000 |
17 | Number of LPS (MW = 10kD) | 600,000 |
18 | Average SA of lipid molecule | 25 Ang2 |
19 | Fraction of lipid bilayer=lipid | 40% |
20 | Fraction of lipid bilayer=protein | 60% |
21 | Number of outer membrane proteins | 300,000 |
22 | Number of porins (subset of OM) | 60,000 |
23 | Number of lipoproteins (OM) | 240,000 |
24 | Number of inner membrane proteins | 200,000 |
25 | Number of nuclear proteins | 100,000 |
26 | Number of cytoplasmic proteins | 1,000,000 (excluding ribo proteins) |
27 | Number of ribosomal proteins | 900,000 |
28 | Number of periplasmic proteins | 80,000 |
29 | Number of all proteins in cell | 2,600,000 |
30 | Number of external proteins (flag/pili) | 1,000,000 |
31 | Number of all proteins | 3,600,000 |
Statistics on Larger Molecule Complexes | ||
1 | Number of protein types to make flagella | 42 |
2 | Length of flagella | 10-20 um or ~15,000 nm |
3 | Diameter of flagella | 25 nm |
4 | Number of protofilaments in flagellum | 11 |
5 | Diameter of each fliC monomer | 5 nm |
6 | Number of fliC monomers in filament | 3000x11=33,000 |
7 | Number of flagella/cell | 10 |
8 | Number of fliC proteins | 330,000 |
9 | Speed at which E. coli move | 50 um/sec = 18 x10-5 km/h |
10 | Number of protein types to make pilus | 1 |
11 | Length of pili/fimbrae | 200-2000 nm |
12 | Diamter of pili | 6.5 nm |
13 | Number of papA/nm pilus | 1.5 |
14 | Number of papA monomers/pilus | 3000-30,000 |
15 | Number of pili/cell | 100-300 |
16 | Number of papA/cell | 300,000-900,000 |
17 | Number of ribosomes/cell | 18,000 |
18 | Number of protein types to make ribosome | 55 |
19 | Number rRNA types to make ribosome | 3 |
20 | Number of proteins in 30S subunit | 21 |
21 | Number of proteins in 50S subunit | 34 |
22 | Number of rRNA in 30S subunit | 1 |
23 | Number of rRNA in 50S subunit | 2 |
24 | Length of all rRNA | 5520 nt |
25 | MW of ribosome | 2700 kD |
26 | MW of RNA component | 1700 kD |
27 | MW of protein component | 1000 kD |
28 | Diameter of ribosome | 20 nm |
29 | Volume of ribosome | 4.2 x 10-24 m3 |
Small Molecule Copy Numbers | ||
1 | Number of water molecules/cell | 2.34x1010 (23.4 billion) |
2 | Number of ions/cell | 120,000,000 (300 mM) |
3 | Number of small organics/cell | 18,000,000 (40-50 mM) |
4 | Number of K ions | 90,000,000 (200-250 mM) |
5 | Number of Na ions | 2,000,000 (5 mM) |
6 | Na (in): Na (out) | 1:20 (in concentration) |
7 | Number of Ca ions | 2,300,000 (6 mM) |
8 | Number of free Ca ions | 40 (100 nM) |
9 | Number of Cl ions | 2,400,000 (6 mM) |
10 | Number of Mg ions | 4,000,000 (10 mM) |
11 | Number of Fe ions | 7,000,000 (18 mM) |
12 | Number of Mn ions | 1,700,000 (4 mM) |
13 | Number of Zn ions | 1,700,000 (4 mM) |
14 | Number of Mo ions | 1,700,000 (4 mM) |
15 | Number of Cu ions | 1,700,000 (4 mM) |
16 | Number of PO4 ions | 2,000,000 (5 mM) |
17 | Number of glucose/cell | 200,000-400,000 (0.5-1 mM) |
18 | Number of PEP/cell | 1,100,000 (2.8 mM) |
19 | Number of pyruvate/cell | 370,000 (0.9 mM) |
20 | Number of gluc-6-PO4/cell | 20,000 (0.05 mM) |
21 | Number of ATP/cell | 500,000 - 3,000,000 (1.3-7.0 mM) |
22 | Number of ADP/cell | 70,000 (0.17 mM) |
23 | Number of NADP/cell | 240,000 (0.63 mM) |
24 | Number of NADPH/cell | 220,000 (0.56 mM) |
25 | Number of all amino acids/cell | 6,000,000 (1.5 mM) |
26 | Number of free Alanine/cell | 350,000 (0.8 mM) |
27 | Number of free Cysteine/cell | 80,000 (0.2 mM) |
28 | Number of free Aspartate/cell | 530,000 (1.34 mM) |
29 | Number of free Glutamate/cell | 200,000 (0.5 mM) |
30 | Number of free Phenylalanine/cell | 170,000 (0.4 mM) |
31 | Number of free Glycine/cell | 350,000 (0.8 mM) |
32 | Number of free Histidine/cell | 80,000 (0.2 mM) |
33 | Number of free Isoleucine/cell | 200,000 (0.5 mM) |
34 | Number of free Lysine/cell | 190,000 (0.46 mM) |
35 | Number of free Leucine/cell | 300,000 (0.7 mM) |
36 | Number of free Methionine/cell | 40,000 (0.1 mM) |
37 | Number of free Asparagine/cell | 200,000 (0.5 mM) |
38 | Number of free Proline/cell | 200,000 (0.5 mM) |
39 | Number of free Glutamine/cell | 200,000 (0.5 mM) |
40 | Number of free Arginine/cell | 170,000 (0.4 mM) |
41 | Number of free Serine/cell | 300,000 (0.7 mM) |
42 | Number of free Threonine/cell | 1,400,000 (3.49 mM) |
43 | Number of free Valine/cell | 240,000 (0.6 mM) |
44 | Number of free Tryptophan/cell | 80,000 (0.2 mM) |
45 | Number of free Tyrosine/cell | 300,000 (0.7 mM) |
46 | Osmotic pressure (pushing out) | 75 lb/in2 |
E. coli Metabolism | ||
1 | 1 glucose generates (total) | 36-38 ATP |
2 | glycolysis yields | 6-8 ATP |
3 | oxidation of pyruvate yields | 6 ATP |
4 | Krebs cycle/e- transport yields | 24 ATP |
5 | Number ATP to make 1 DNA | 72,289,000 |
6 | Number ATP to make 1 protein (360 aa) | 1500 |
7 | Number ATP to make 1 lipid | 7 |
8 | Number ATP to make 1 polysaccharide | 2000 |
9 | Number ATP to make 1 RNA (1000 nt) | 2000 |
10 | Number ATP to make 1 cell | 55 billion ATP |
11 | Number Glucose molecules consumed | 1.4 billion molecules |
12 | Cell division rate | 1 division/30 minutes |
Wednesday, April 20, 2011
PSLV - C 16 launch successfully
India’s PSLV—C16 rocket April 2o, successfully launched into orbit the latest remote sensing satellite Resourcesat—2 that would study and help manage natural resources along with two nano satellites.
ISRO’s homegrown workhorse Polar Satellite Launch Vehicle placed in a ‘Polar Sun Synchronous Orbit’ Resourcesat-2, Youthsat and X-Sat about 18 minutes after it blasted off from the Satish Dhawan Space Centre launch pad at 10.12 am.
“PSLV-C16 Resourcesat-2 mission is successful,” a jubilant Indian Space Research Organisation Chairman K Radhakrishnan announced shortly after all the three satellites were hurled into space one after another 822 km above earth in a text book launch.
The ISRO chief’s announcement was cheered by the battery of scientists at the mission control centre who heaved a sigh of relief as they were gripped by an added anxiety following two successive failures of GSLV missions last year.
The 1,206 kg Resourcesat-2 with a space life of five years replaces Resourcesat-1 launched in 2003 and would provide data with enhanced multispectral and spatial coverage on natural resources.
The GSLV mission in December last year failed when the homegrown GSLV F06 carrying communication satellite GSAT-5P exploded mid-air less than a minute after lift-off and fell into the Bay of Bengal.
GSAT-5P, carrying 24 C-band and 12 extended C-band transponders, plunged into the sea when the destruct command was issued as the rocket veered from its flight path.
Earlier, the GSLV-D3 mission carrying GSAT-4 had also failed in April 2010, dealing a blow to India’s space programme.
PSLV—C16 flight was its 17th successive mission after the failure of its maiden voyage in September 1993.
PSLV-C16 Details:
PSLV-C16, is the eighteenth flight of ISRO's Polar Satellite Launch Vehicle, PSLV. In this flight, the standard version of PSLV with six solid strap-on motors is used.
PSLV-C16 will place three satellites with a total payload mass of 1404 kg - RESOURCESAT-2 weighing 1206 kg, the Indo-Russian YOUTHSAT weighing 92 kg and Singapore's X-SAT weighing 106 kg – into an 822 km polar Sun Synchronous Orbit (SSO). PSLV-C16 will be launched from the First Launch Pad (FLP) at Satish Dhawan Space Centre SHAR, Sriharikota.
The major changes made in PSLV since its first launch include changes in strap-on motors ignition sequence, increase in the propellant loading of the first stage and strap-on solid propellant motors as well as the second and fourth stage liquid propellant motors, improvement in the performance of the third stage motor by optimising motor case and enhanced propellant loading and employing a carbon composite payload adapter.
PSLV has also become a more versatile vehicle for launching multiple satellites in polar SSOs as well as Low Earth Orbits (LEO) and Geosynchronous Transfer Orbit (GTO). With sixteen successful launches, PSLV has emerged as the workhorse launch vehicle of ISRO and is offered for launching satellites for international customers also. During 1994-2010 period, PSLV has launched a total of 44 satellites, of which 25 satellites are from abroad and 19 are Indian satellites.
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RESOURCESAT-2: RESOURCESAT-2 is a follow on mission to RESOURCESAT-1 and the eighteenth Remote Sensing satellite built by ISRO. RESOURCESAT-2 is intended to continue the remote sensing data services to global users provided by RESOURCESAT-1, and to provide data with enhanced multispectral and spatial coverage as well. Important changes in RESOURCESAT-2 compared to RESOURCESAT-1 are: Enhancement of LISS-4 multispectral swath from 23 km to 70 km and improved Radiometric accuracy from 7 bits to 10 bits for LISS-3 and LISS-4 and 10 bits to 12 bits for AWIFS. Besides, suitable changes, including miniaturisation in payload electronics, have been made in RESOURCESAT-2. RESOURCESAT-2 also carries an additional payload known as AIS (Automatic Identification System) from COMDEV, Canada as an experimental payload for ship surveillance in VHF band to derive position, speed and other information about ships. RESOURCESAT-2 carries two Solid State Recorders with a capacity of 200 Giga Bytes each to store the images taken by its cameras which can be read out later to ground stations. | |||||||||||||||||||||||||||||||||
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YOUTHSAT: YOUTHSAT is a joint Indo-Russian stellar and atmospheric satellite mission with the participation of students from Universities at graduate, post graduate and research scholar level. With a lift-off mass of 92 kg, Youthsat is a mini satellite and the second in the Indian Mini Satellite (IMS) series. Youthsat mission intends to investigate the relationship between solar variability and thermosphere-Ionosphere changes. The satellite carries three payloads, of which two are Indian and one Russian. Together, they form a unique and comprehensive package of experiments for the investigation of the composition, energetics and dynamics of earth's upper atmosphere. The Indian payloads are:
SOLRAD - For monitoring the solar X- and gamma ray fluxes and to study solar cosmic ray flux parameters and conditions of their penetration in the Earth's magnetosphere. | |||||||||||||||||||||||||||||
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X-SAT:
X-SAT, the third payload of PSLV-C16, is Singapore's first satellite. Weighing 106 kg at lift-off, X-SAT is a Mini Satellite with a multispectral camera IRIS as its primary payload. X-SAT mission mainly intends to demonstrate technologies related to satellite based remote sensing and onboard image processing.
ISRO MILESTONES
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